- Benefits of Solid Phase Extraction -
Solid Phase Extraction (SPE) is widely used for the purification of small compound libraries synthezised in solution. Compared to the classic liquid-liquid extraction using the separatory funnel or
the preparative HPLC purification it offers several advantages:
- Reduced lab time
- Easy manipulation
- Lesser amount of solvent required, no disposal of large quantaties of organic solvents
- Higher concentration factor
- No problem with the miscibility of solvent
- Easy adaptable for very selective extraction
- Easy automatisation
- Avoids problems such as incomplete phase separations, less-than-quantitative recoveries and emulsion formation as encountered in liquid-liquid extractions
Reaction mixtures from parallel synthesis can easily be purified in a parallel fashion with commercially available disposable cartridges/tubes/disks and a vacuum manifold.
However, a basic knowledge is necessary of how to perform SPE extractions in a efficient way by selecting the right sorbent (from a variety of reversed, normal or ion exchange phases),
conditioning, selective washing and eluting the compounds of interest.
- Directory Contents -
The web site "Solid Phase Extraction in Organic Synthesis" is an excellent starting point for both a researcher new to the field as an expert who is looking for a intuitively organized web site about this subject.
Contents:
- Introduction (6 listings)
- Sorbent Suppliers (10 listings)
- Vacuum Manifolds (5 listings)
- Automated Systems (14 listings)
- Recent Reviews (5 listings)
- Recent Books (4 listings)
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